pbabe zeo large t genomic plasmid Search Results


pbabe neo sv40 large t  (Addgene inc)
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Pbabe Neo Sv40 Large T, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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large t antigen pbabe sv40 large t antigen  (Addgene inc)
94
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Large T Antigen Pbabe Sv40 Large T Antigen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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large t antigen  (Addgene inc)
93
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Large T Antigen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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retrovirus  (Addgene inc)
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Average 93 stars, based on 1 article reviews
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sv40 t antigen  (Addgene inc)
93
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Sv40 T Antigen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbabe gfp small t antigen  (Addgene inc)
88
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pbabe neo larget vectors  (Addgene inc)
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Pbabe Neo Larget Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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pbabe zeo sv40 large t genomic dna  (Addgene inc)
92
Addgene inc pbabe zeo sv40 large t genomic dna
Figure 1. TE expression increases at the subfamily level during transformation. A, Model schematic. Early passage BJ fibroblast cells (EP) were sequentially infected with retrovirus packaged with pBabe-hygro-hTERT (hTERT), <t>pBabe-zeo-SV40</t> large T <t>genomic</t> <t>DNA</t> (LT and ST antigens; SV40), and pBabe-puro-HrasV12 (HRAS). B, Number of significantly differentially expressed TE subfamilies at each stage of transformation (Padj < 0.05, fold change greater than 2 or less than 2). Teal, upregulated elements; violet, downregulated elements. C, Class composition of significantly up- and downregulated elements (Padj < 0.05, fold change greater than 2 or less than 2). Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. D, Overlap of upregulated elements in B between stages of transformation. Yellow, hTERT; red, SV40, blue, HRAS. E, Volcano plot showing log2-fold change between each stage and EP vs. log10 Padj. Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. Gray lines indicate Padj ¼ 0.05 and fold change ¼ 2 and 2. F, Fold change increase in LTR26 by qPCR. Pink, EP; yellow, hTERT; red, SV40; blue, HRAS. Error bars, SEM. Asterisks represent statistically significant differences. , P < 0.05; , P < 0.01. N ¼ 3 for each sample.
Pbabe Zeo Sv40 Large T Genomic Dna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbabe hygro htert 1773 psg5 large t 9053  (Addgene inc)
93
Addgene inc pbabe hygro htert 1773 psg5 large t 9053
Figure 1. TE expression increases at the subfamily level during transformation. A, Model schematic. Early passage BJ fibroblast cells (EP) were sequentially infected with retrovirus packaged with pBabe-hygro-hTERT (hTERT), <t>pBabe-zeo-SV40</t> large T <t>genomic</t> <t>DNA</t> (LT and ST antigens; SV40), and pBabe-puro-HrasV12 (HRAS). B, Number of significantly differentially expressed TE subfamilies at each stage of transformation (Padj < 0.05, fold change greater than 2 or less than 2). Teal, upregulated elements; violet, downregulated elements. C, Class composition of significantly up- and downregulated elements (Padj < 0.05, fold change greater than 2 or less than 2). Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. D, Overlap of upregulated elements in B between stages of transformation. Yellow, hTERT; red, SV40, blue, HRAS. E, Volcano plot showing log2-fold change between each stage and EP vs. log10 Padj. Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. Gray lines indicate Padj ¼ 0.05 and fold change ¼ 2 and 2. F, Fold change increase in LTR26 by qPCR. Pink, EP; yellow, hTERT; red, SV40; blue, HRAS. Error bars, SEM. Asterisks represent statistically significant differences. , P < 0.05; , P < 0.01. N ¼ 3 for each sample.
Pbabe Hygro Htert 1773 Psg5 Large T 9053, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pentr1a plasmid  (Addgene inc)
91
Addgene inc pentr1a plasmid
Figure 1. TE expression increases at the subfamily level during transformation. A, Model schematic. Early passage BJ fibroblast cells (EP) were sequentially infected with retrovirus packaged with pBabe-hygro-hTERT (hTERT), <t>pBabe-zeo-SV40</t> large T <t>genomic</t> <t>DNA</t> (LT and ST antigens; SV40), and pBabe-puro-HrasV12 (HRAS). B, Number of significantly differentially expressed TE subfamilies at each stage of transformation (Padj < 0.05, fold change greater than 2 or less than 2). Teal, upregulated elements; violet, downregulated elements. C, Class composition of significantly up- and downregulated elements (Padj < 0.05, fold change greater than 2 or less than 2). Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. D, Overlap of upregulated elements in B between stages of transformation. Yellow, hTERT; red, SV40, blue, HRAS. E, Volcano plot showing log2-fold change between each stage and EP vs. log10 Padj. Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. Gray lines indicate Padj ¼ 0.05 and fold change ¼ 2 and 2. F, Fold change increase in LTR26 by qPCR. Pink, EP; yellow, hTERT; red, SV40; blue, HRAS. Error bars, SEM. Asterisks represent statistically significant differences. , P < 0.05; , P < 0.01. N ¼ 3 for each sample.
Pentr1a Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. TE expression increases at the subfamily level during transformation. A, Model schematic. Early passage BJ fibroblast cells (EP) were sequentially infected with retrovirus packaged with pBabe-hygro-hTERT (hTERT), pBabe-zeo-SV40 large T genomic DNA (LT and ST antigens; SV40), and pBabe-puro-HrasV12 (HRAS). B, Number of significantly differentially expressed TE subfamilies at each stage of transformation (Padj < 0.05, fold change greater than 2 or less than 2). Teal, upregulated elements; violet, downregulated elements. C, Class composition of significantly up- and downregulated elements (Padj < 0.05, fold change greater than 2 or less than 2). Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. D, Overlap of upregulated elements in B between stages of transformation. Yellow, hTERT; red, SV40, blue, HRAS. E, Volcano plot showing log2-fold change between each stage and EP vs. log10 Padj. Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. Gray lines indicate Padj ¼ 0.05 and fold change ¼ 2 and 2. F, Fold change increase in LTR26 by qPCR. Pink, EP; yellow, hTERT; red, SV40; blue, HRAS. Error bars, SEM. Asterisks represent statistically significant differences. , P < 0.05; , P < 0.01. N ¼ 3 for each sample.

Journal: Cancer Research

Article Title: Oncogenic Transformation Drives DNA Methylation Loss and Transcriptional Activation at Transposable Element Loci

doi: 10.1158/0008-5472.can-22-3485

Figure Lengend Snippet: Figure 1. TE expression increases at the subfamily level during transformation. A, Model schematic. Early passage BJ fibroblast cells (EP) were sequentially infected with retrovirus packaged with pBabe-hygro-hTERT (hTERT), pBabe-zeo-SV40 large T genomic DNA (LT and ST antigens; SV40), and pBabe-puro-HrasV12 (HRAS). B, Number of significantly differentially expressed TE subfamilies at each stage of transformation (Padj < 0.05, fold change greater than 2 or less than 2). Teal, upregulated elements; violet, downregulated elements. C, Class composition of significantly up- and downregulated elements (Padj < 0.05, fold change greater than 2 or less than 2). Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. D, Overlap of upregulated elements in B between stages of transformation. Yellow, hTERT; red, SV40, blue, HRAS. E, Volcano plot showing log2-fold change between each stage and EP vs. log10 Padj. Yellow, DNA elements; gray, LINE elements; light blue, LTR elements; dark blue, other. Other includes RC, satellite, snRNA, tRNA, and unknown. Gray lines indicate Padj ¼ 0.05 and fold change ¼ 2 and 2. F, Fold change increase in LTR26 by qPCR. Pink, EP; yellow, hTERT; red, SV40; blue, HRAS. Error bars, SEM. Asterisks represent statistically significant differences. , P < 0.05; , P < 0.01. N ¼ 3 for each sample.

Article Snippet: Briefly, early passage BJ fibroblast cells (EP) were sequentially infected with retrovirus packaged with pBabe-hygro-hTERT (RRID:Addgene_1773), pBabe-zeo-SV40 large T genomic DNA (LT and ST antigens; RRID:Addgene_1778) and pBabe-puroHrasV12 (RRID:Addgene_1768).

Techniques: Expressing, Transformation Assay, Infection